Open AccessDirect Fluorescent-Antibody Testing Followed by Culture for Diagnosis of 2009 H1N1 Influenza A
Author(s) -
Paul R. Bakerman,
Lilanthi Balasuriya,
Ora Fried,
David Téllez,
Pamela GarciaFilion,
Heidi J. Dalton
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.05255-11
Subject(s) - virology , direct fluorescent antibody , antibody , fluorescence , medicine , microbiology and biotechnology , influenza a virus , immunology , biology , virus , physics , quantum mechanics
During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent-antibody testing, which missed one-third of infected individuals.
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