Prevalence of Shiga Toxin-Producing Escherichia coli as Detected by Enzyme-Linked Immunoassays and Real-Time PCR during the Summer Months in Northern Alberta, Canada | Zendy

Linda Chui | Zendy; Mao-Cheng Lee | Zendy; Kathy Malejczyk | Zendy; Lillian Yuxian Lim | Zendy; Daniel Fok | Zendy; Pauline Kwong | Zendy

Open Access

Prevalence of Shiga Toxin-Producing Escherichia coli as Detected by Enzyme-Linked Immunoassays and Real-Time PCR during the Summer Months in Northern Alberta, Canada

Author(s) -

Linda Chui,

Mao-Cheng Lee,

Kathy Malejczyk,

Lillian Yuxian Lim,

Daniel Fok,

Pauline Kwong

Publication year - 2011

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.05211-11

Subject(s) - escherichia coli , microbiology and biotechnology , enterobacteriaceae , toxin , diarrhea , shiga toxin , feces , shiga like toxin , biology , medicine , gene , biochemistry

Shiga toxin-producingEscherichia coli (STEC) in northern Alberta was detected using two enzyme immunoassays and an in-house real-time PCR. Of 2,328 stool samples, 8 were positive for O157:H7 STEC and 13 were positive for non-O157 STEC. No significant gender (P = 0.17) or age (P = 0.81) differences between groups were seen. Most positive diarrheal stool samples were nonbloody.

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