Open AccessDried Culture Spots for Xpert MTB/RIF External Quality Assessment: Results of a Phase 1 Pilot Study in South Africa
Author(s) -
Lesley Scott,
Natasha Gous,
Brad Cunningham,
Bavesh D Kana,
Olga Perovic,
Linda Erasmus,
Gerrit Coetzee,
H. J. Koornhof,
Wendy Stevens
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.05167-11
Subject(s) - mycobacterium tuberculosis , tuberculosis , external quality assessment , microbiology and biotechnology , medicine , spots , virology , quality assessment , biology , pathology
Implementation of Xpert MTB/RIF requires quality assessment. A pilot program using dried culture spots (DCSs) of inactivated Mycobacterium tuberculosis is described. Of 274 DCS results received, 2.19% generated errors; the remainder yielded 100% correct Mycobacterium tuberculosis detection. The probe A cycle threshold (C(T)) variability of three DCS batches was ≤ 3.47. The study of longer-term DCS stability is ongoing.
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