Evaluation of a Short, On-Plate Formic Acid Extraction Method for Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry-Based Identification of Clinically Relevant Yeast Isolates | Zendy

Rebecca Gorton | Zendy; Shila Seaton | Zendy; P. Ramnarain | Zendy; Timothy D. McHugh | Zendy; Christopher C. Kibbler | Zendy

Open Access

Evaluation of a Short, On-Plate Formic Acid Extraction Method for Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry-Based Identification of Clinically Relevant Yeast Isolates

Author(s) -

Rebecca Gorton,

Shila Seaton,

P. Ramnarain,

Timothy D. McHugh,

Christopher C. Kibbler

Publication year - 2014

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03489-13

Subject(s) - formic acid , mass spectrometry , extraction (chemistry) , chromatography , matrix assisted laser desorption/ionization , matrix (chemical analysis) , desorption , chemistry , identification (biology) , analytical chemistry (journal) , biology , organic chemistry , adsorption , botany

This report describes a short, on-plate formic acid (FA) extraction method for the identification of clinical yeast isolates using matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS). A total of 41.1% (78/190) and 63.7% (121/190) of yeasts were identified using species log score thresholds of >2.0 and >1.9, respectively. Overall, 97.4% (185/190) of yeasts were identified in combination with conventional FA extraction.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research