Development of a Peptide Nucleic Acid-Based Multiprobe Real-Time PCR Method Targeting the hsp65 Gene for Differentiation among Mycobacterium abscessus Strains | Zendy

Kijeong Kim | Zendy; Byoung-Jun Kim | Zendy; Tae Sun Shim | Zendy; Seok-Hyun Hong | Zendy; YoonHoh Kook | Zendy; BumJoon Kim | Zendy

Open Access

Development of a Peptide Nucleic Acid-Based Multiprobe Real-Time PCR Method Targeting the hsp65 Gene for Differentiation among Mycobacterium abscessus Strains

Author(s) -

Kijeong Kim,

Byoung-Jun Kim,

Tae Sun Shim,

Seok-Hyun Hong,

YoonHoh Kook,

BumJoon Kim

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03424-14

Subject(s) - mycobacterium abscessus , peptide nucleic acid , biology , mycobacterium , nucleic acid , gene , microbiology and biotechnology , subspecies , computational biology , genetics , bacteria , paleontology

Recently, the need to distinguish between members of the Mycobacterium abscessus group has gained increasing attention. Here, we introduced a novel peptide nucleic acid (PNA) real-time PCR method targeting the hsp65 gene in order to distinguish between four subspecies within the M. abscessus group (M. abscessus and 3 types of M. massiliense).

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