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Recently, the need to distinguish between members of the Mycobacterium abscessus group has gained increasing attention. Here, we introduced a novel peptide nucleic acid (PNA) real-time PCR method targeting the hsp65 gene in order to distinguish between four subspecies within the M. abscessus group (M. abscessus and 3 types of M. massiliense).

journal of clinical microbiology

Development of a Peptide Nucleic Acid-Based Multiprobe Real-Time PCR Method Targeting the <i>hsp65</i> Gene for Differentiation among Mycobacterium abscessus Strains

Development of a Peptide Nucleic Acid-Based Multiprobe Real-Time PCR Method Targeting the hsp65 Gene for Differentiation among Mycobacterium abscessus Strains