Real-Time PCR Assay for Detection of blaZ Genes in Staphylococcus aureus Clinical Isolates
Author(s) -
Lynette A. Pereira,
Gerald B. Harnett,
Meredith Hodge,
Julia A. Cattell,
David Speers
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.03413-13
Subject(s) - staphylococcus aureus , gene , biology , microbiology and biotechnology , polymerase chain reaction , staphylococcal infections , real time polymerase chain reaction , phenotype , staphylococcus , virology , genetics , bacteria
The Clinical and Laboratory Standards Institute recommends consideration of blaZ gene testing for cases of serious Staphylococcus aureus infection. Conventional PCR methods have demonstrated superior sensitivity and specificity to phenotypic tests. To our knowledge, this is the first description of real-time PCR detection of the blaZ gene.
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