Development of a Two-Step Multiplex PCR Assay for Typing of Capsular Polysaccharide Synthesis Gene Clusters of Streptococcus suis | Zendy

Masatoshi Okura | Zendy; Claude Lachance | Zendy; Makoto Osaki | Zendy; Tsutomu Sekizaki | Zendy; Fumito Maruyama | Zendy; Takashi Nozawa | Zendy; Ichirô Nakagawa | Zendy; Shigeyuki Hamada | Zendy; Céline Rossignol | Zendy; Marcelo Gottschalk | Zendy; Daisuke Takamatsu | Zendy

Open Access

Development of a Two-Step Multiplex PCR Assay for Typing of Capsular Polysaccharide Synthesis Gene Clusters of Streptococcus suis

Author(s) -

Masatoshi Okura,

Claude Lachance,

Makoto Osaki,

Tsutomu Sekizaki,

Fumito Maruyama,

Takashi Nozawa,

Ichirô Nakagawa,

Shigeyuki Hamada,

Céline Rossignol,

Marcelo Gottschalk,

Daisuke Takamatsu

Publication year - 2014

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03411-13

Subject(s) - streptococcus suis , multiplex polymerase chain reaction , multiplex , typing , biology , microbiology and biotechnology , streptococcaceae , polymerase chain reaction , gene , genetics , antibiotics , virulence

We developed a practical and easy two-step multiplex PCR assay to aid in serotyping of Streptococcus suis. The assay accurately typed almost all of the serotype reference strains and field isolates of various serotypes and also identified the genotypes of capsular polysaccharide synthesis gene clusters of some serologically nontypeable strains.

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