Development of a Luminex-Based Multiplex Assay for Detection of Mutations Conferring Resistance to Echinocandins in Candida glabrata

Echinocandins are the recommended treatment for invasive candidiasis due toCandida glabrata . Resistance to echinocandins is known to be caused by nonsynonymous mutations in the hot spot-1 (HS1) regions of theFKS1 andFKS2 genes, which encode a subunit of the β-1,3-glucan synthase, the target of echinocandins. Here, we describe the development of a microsphere-based assay using Luminex MagPix technology to identify mutations in theFKS1 HS1 andFKS2 HS1 domains, which conferin vitro echinocandin resistance inC. glabrata isolates. The assay is rapid and can be performed with high throughput. The assay was validated using 102 isolates that hadFKS1 HS1 andFKS2 HS1 domains previously characterized by DNA sequencing. The assay was 100% concordant with DNA sequencing results. The assay was then used for high-throughput screening of 1,032C. glabrata surveillance isolates. Sixteen new isolates with mutations, including a mutation that was new to our collection (del659F), were identified. This assay provides a rapid and cost-effective way to screenC. glabrata isolates for echinocandin resistance.