Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients | Zendy

Sylvie Zida | Zendy; Édouard Tuaillon | Zendy; Makoura Barro | Zendy; Arnaud Kwimatouo Lekpa Franchard | Zendy; Thérèse Kagoné | Zendy; Boubacar Nacro | Zendy; Abdoul–Salam Ouédraogo | Zendy; Karine Bolloré | Zendy; Armen Sanosyan | Zendy; JeanChristophe Plantier | Zendy; Nicolas Méda | Zendy; Lassana Sangaré | Zendy; Christine Rouzioux | Zendy; François Rouet | Zendy; Dramane Kania | Zendy

Open Access

Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

Author(s) -

Sylvie Zida,

Édouard Tuaillon,

Makoura Barro,

Arnaud Kwimatouo Lekpa Franchard,

Thérèse Kagoné,

Boubacar Nacro,

Abdoul–Salam Ouédraogo,

Karine Bolloré,

Armen Sanosyan,

JeanChristophe Plantier,

Nicolas Méda,

Lassana Sangaré,

Christine Rouzioux,

François Rouet,

Dramane Kania

Publication year - 2016

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03372-15

Subject(s) - dried blood , rna , peripheral blood mononuclear cell , dna , human immunodeficiency virus (hiv) , dried blood spot , spots , virology , biology , peripheral blood , immunology , chemistry , gene , chromatography , in vitro , genetics , botany

The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/106 peripheral blood mononuclear cells.

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