A Real-Time PCR Assay for Rapid Detection and Quantification of Exserohilum rostratum, a Causative Pathogen of Fungal Meningitis Associated with Injection of Contaminated Methylprednisolone | Zendy

Yanan Zhao | Zendy; Rūta Petraitienė | Zendy; Thomas J. Walsh | Zendy; David S. Perlin | Zendy

Open Access

A Real-Time PCR Assay for Rapid Detection and Quantification of Exserohilum rostratum, a Causative Pathogen of Fungal Meningitis Associated with Injection of Contaminated Methylprednisolone

Author(s) -

Yanan Zhao,

Rūta Petraitienė,

Thomas J. Walsh,

David S. Perlin

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03369-12

Subject(s) - exserohilum , microbiology and biotechnology , biology , methylprednisolone acetate , meningitis , real time polymerase chain reaction , pathogen , fungal pathogen , virology , gene , medicine , genetics , botany , blight , psychiatry , corticosteroid , endocrinology

A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis ofExserohilum rostratum infection. As low as 100 fg ofE. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research