Is Supplementary Bead Beating for DNA Extraction from Nematode Eggs by Use of the NucliSENS easyMag Protocol Necessary?
Author(s) -
Lee O’Brien Andersen,
Dennis Röser,
Peter Nejsum,
Henrik Nielsen,
Christen Rune Stensvold
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.03353-12
Subject(s) - bead , nematode , dna extraction , biology , virology , extraction (chemistry) , dna , genetics , geography , polymerase chain reaction , ecology , chromatography , chemistry , archaeology , gene
Recent studies have demonstrated the effectiveness and applicability of PCR-based methods for the detection of intestinal helminths in stool samples ([1][1][–][2][5][3]); however, successful DNA extraction is essential. We compared DNA extractions using NucliSENS easyMag, with and without a
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