Open AccessReal-Time PCR Assay for Rapid Detection of Epidemiologically and Clinically Significant Mycobacterium tuberculosis Beijing Genotype Isolates
Author(s) -
Igor Mokrousov,
Anna Vyazovaya,
Viacheslav Zhuravlev,
Tatiana Otten,
Julie Millet,
Weiwei Jiao,
Adong Shen,
Nalin Rastogi,
Boris Vishnevsky,
Olga Narvskaya
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.03193-13
Subject(s) - genotype , mycobacterium tuberculosis , virology , beijing , biology , tuberculosis , polymerase chain reaction , microbiology and biotechnology , genetics , medicine , gene , geography , china , pathology , archaeology
Mycobacterium tuberculosis Beijing genotype strains are rapidly disseminating, frequently hypervirulent, and multidrug resistant. Here, we describe a method for their rapid detection by real-time PCR that targets the specific IS6110 insertion in the dnaA-dnaN genome region. The method was evaluated with a geographically and genetically diverse collection representing areas in East Asia and the former Soviet Union in which the Beijing genotype is endemic and epidemic (i.e., major foci of its global propagation) and with clinical specimens.
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