Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR | Zendy

Eva Malatinková | Zendy; Maja Kiselinova | Zendy; Pawel Bonczkowski | Zendy; Wim Trypsteen | Zendy; Peter Messiaen | Zendy; Jolien Vermeíre | Zendy; Bruno Verhasselt | Zendy; Karen Vervisch | Zendy; Linos Vandekerckhove | Zendy; Ward De Spiegelaere | Zendy

Open Access

Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR

Author(s) -

Eva Malatinková,

Maja Kiselinova,

Pawel Bonczkowski,

Wim Trypsteen,

Peter Messiaen,

Jolien Vermeíre,

Bruno Verhasselt,

Karen Vervisch,

Linos Vandekerckhove,

Ward De Spiegelaere

Publication year - 2014

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03087-14

Subject(s) - digital polymerase chain reaction , long terminal repeat , plasmid , biology , dna , isolation (microbiology) , human immunodeficiency virus (hiv) , microbiology and biotechnology , virology , computational biology , polymerase chain reaction , genetics , genome , bioinformatics , gene

Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation.

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