Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves | Zendy

Bobby L. Boyanton | Zendy; Amro Almradi | Zendy; Mark J. Espy | Zendy; Anne E. Prada | Zendy; John P. Gibson | Zendy; Bobbi S. Pritt | Zendy

Open Access

Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves

Author(s) -

Bobby L. Boyanton,

Amro Almradi,

Mark J. Espy,

Anne E. Prada,

John P. Gibson,

Bobbi S. Pritt

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03062-13

Subject(s) - analyte , melting curve analysis , reagent , standard curve , area under curve , biology , chromatography , microbiology and biotechnology , chemistry , real time polymerase chain reaction , genetics , pharmacokinetics , bioinformatics , gene

We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.

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