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We present a duplex, real-time PCR assay for detection ofKlebsiella pneumoniae carbapenemase (bla KPC ) and New Delhi metallo-β-lactamase (bla NDM ) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

journal of clinical microbiology

Rapid and Simultaneous Detection of Genes Encoding Klebsiella pneumoniae Carbapenemase ( bla KPC ) and New Delhi Metallo-β-Lactamase ( bla NDM ) in Gram-Negative Bacilli