Rapid and Simultaneous Detection of Genes Encoding Klebsiella pneumoniae Carbapenemase ( bla KPC ) and New Delhi Metallo-β-Lactamase ( bla NDM ) in Gram-Negative Bacilli | Zendy

Scott A. Cunningham | Zendy; Tabassum Noorie | Zendy; Danièle Meunier | Zendy; Neil Woodford | Zendy; Robin Patel | Zendy

Open Access

Rapid and Simultaneous Detection of Genes Encoding Klebsiella pneumoniae Carbapenemase ( bla _KPC ) and New Delhi Metallo-β-Lactamase ( bla _NDM ) in Gram-Negative Bacilli

Author(s) -

Scott A. Cunningham,

Tabassum Noorie,

Danièle Meunier,

Neil Woodford,

Robin Patel

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.03062-12

Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , bacilli , biology , new delhi , klebsiella infections , enterobacteriaceae , gene , bacterial protein , klebsiella , gram , bacteria , escherichia coli , medicine , genetics , metropolitan area , pathology

We present a duplex, real-time PCR assay for detection ofKlebsiella pneumoniae carbapenemase (bla KPC ) and New Delhi metallo-β-lactamase (bla NDM ) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

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