Verification of a Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Diagnostic Identification of High-Consequence Bacterial Pathogens | Zendy

Dobryan M. Tracz | Zendy; Kym Antonation | Zendy; Cindi R. Corbett | Zendy

Open Access

Verification of a Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Diagnostic Identification of High-Consequence Bacterial Pathogens

Author(s) -

Dobryan M. Tracz,

Kym Antonation,

Cindi R. Corbett

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02709-15

Subject(s) - mass spectrometry , identification (biology) , matrix assisted laser desorption/ionization , chromatography , time of flight mass spectrometry , ionization , microbiology and biotechnology , chemistry , desorption , biology , organic chemistry , adsorption , ion , botany

We examined the utility of a single matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry method for the identification of security-sensitive biological agents (risk group 3 bacterial pathogens). The goal was 2-fold: to verify a method for inclusion into our scope of accreditation, and to assess the biological safety of extractions. We developed our sample flow to include a tube-based chemical extraction, followed by filtration, before processing on MALDI-TOF MS instruments in a containment level 2 laboratory.

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