Open AccessEfficient Direct Extended-Spectrum β-Lactamase Detection by Multiplex Real-Time PCR: Accurate Assignment of Phenotype by Use of a Limited Set of Genetic Markers
Author(s) -
Justin Ellem,
Sally R. Partridge,
Jonathan R. Iredell
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02647-10
Subject(s) - multiplex , biology , multiplex polymerase chain reaction , genetics , gene , phenotype , enterobacteriaceae , computational biology , polymerase chain reaction , escherichia coli
The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum β-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum β-lactamase (ESBL)-producingEnterobacteriaceae .