Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR | Zendy

L. Mia Kim | Zendy; Daniel J. King | Zendy; David L. Suarez | Zendy; Chun Wai Wong | Zendy; Claudio L. Afonso | Zendy

Open Access

Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR

Author(s) -

L. Mia Kim,

Daniel J. King,

David L. Suarez,

Chun Wai Wong,

Claudio L. Afonso

Publication year - 2007

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02594-06

Subject(s) - newcastle disease , biology , virology , virus , gene , phylogenetic tree , reverse transcription polymerase chain reaction , real time polymerase chain reaction , reverse transcriptase , genetics , polymerase chain reaction , gene expression

Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.

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