Glycoprotein-Based Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Infectious Laryngotracheitis | Zendy

Mallikarjuna Kanabagatte Basavarajappa | Zendy; Haichen Song | Zendy; Chinta Lamichhane | Zendy; Siba K. Samal | Zendy

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Glycoprotein-Based Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Infectious Laryngotracheitis

Author(s) -

Mallikarjuna Kanabagatte Basavarajappa,

Haichen Song,

Chinta Lamichhane,

Siba K. Samal

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02540-14

Subject(s) - virology , glycoprotein , seroconversion , antibody , newcastle disease , recombinant dna , biology , virus , microbiology and biotechnology , immunology , biochemistry , gene

For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with vectored vaccine, using recombinant Newcastle disease virus (rNDV) expressing glycoprotein D (gD) as the vaccine vector.

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