Open AccessEvaluation of High-Resolution Melting Curve Analysis of Ligation-Mediated Real-Time PCR, a Rapid Method for Epidemiological Typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) Pathogens
Author(s) -
Hanna Woksepp,
Anna Ryberg,
Hanna Billström,
Anita Hällgren,
Lennart Nilsson,
BrittInger Marklund,
B Olsson-Liljequist,
Thomas Schön
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02537-14
Subject(s) - enterococcus faecium , acinetobacter baumannii , microbiology and biotechnology , klebsiella pneumoniae , pseudomonas aeruginosa , pulsed field gel electrophoresis , staphylococcus aureus , biology , typing , klebsiella oxytoca , acinetobacter , virology , antibiotics , bacteria , escherichia coli , genotype , biochemistry , genetics , gene
A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing ofEnterococcus faecium ,Staphylococcus aureus ,Klebsiella pneumoniae ,Acinetobacter baumannii ,Pseudomonas aeruginosa , andEnterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.
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