Rapid Detection of OXA-48-Producing Enterobacteriaceae by Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry | Zendy

Marina Oviaño | Zendy; María José Barba | Zendy; B. Fernández | Zendy; Adriana Ortega | Zendy; Belén Aracil | Zendy; Jesús Oteo | Zendy; José Campos | Zendy; Germán Bou | Zendy

Open Access

Rapid Detection of OXA-48-Producing Enterobacteriaceae by Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry

Author(s) -

Marina Oviaño,

María José Barba,

B. Fernández,

Adriana Ortega,

Belén Aracil,

Jesús Oteo,

José Campos,

Germán Bou

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02496-15

Subject(s) - ertapenem , mass spectrometry , enterobacteriaceae , matrix assisted laser desorption/ionization , chromatography , time of flight mass spectrometry , chemistry , matrix (chemical analysis) , desorption , surface enhanced laser desorption/ionization , microbiology and biotechnology , analytical chemistry (journal) , ionization , biology , tandem mass spectrometry , escherichia coli , biochemistry , ion , protein mass spectrometry , organic chemistry , adsorption , gene

A rapid and sensitive (100%) matrix-assisted laser desorption ionization−time of flight mass spectrometry (MALDI-TOF MS) assay was developed to detect OXA-48-type producers, using 161 previously characterized clinical isolates. Ertapenem was monitored to detect carbapenem resistance, and temocillin was included in the assay as a marker for OXA-48-producers. Structural analysis of temocillin is described. Data are obtained within 60 min.

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