Multiplex Real-Time PCR Assay for Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Suitable in Regions of High MRSA Endemicity | Zendy

Jeong-Uk Kim | Zendy; ChoongHwan Cha | Zendy; Hae-Kyong An | Zendy; Hojun Lee | Zendy; MiNa Kim | Zendy

Open Access

Multiplex Real-Time PCR Assay for Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Suitable in Regions of High MRSA Endemicity

Author(s) -

Jeong-Uk Kim,

ChoongHwan Cha,

Hae-Kyong An,

Hojun Lee,

MiNa Kim

Publication year - 2012

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02495-12

Subject(s) - sccmec , staphylococcus aureus , microbiology and biotechnology , multiplex , biology , methicillin resistant staphylococcus aureus , multiplex polymerase chain reaction , staphylococcal infections , virology , polymerase chain reaction , micrococcaceae , gene , bacteria , genetics

A multiplex real-time PCR assay that simultaneously detects themecA , staphylococcal cassette chromosome (SCCmec )-open reading frame X (orfX ) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistantStaphylococcus aureus (MRSA) infections.

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