Monitoring of Patients Supported by Extracorporeal Membrane Oxygenation for Systemic Infections by Broad-Range rRNA Gene PCR Amplification and Sequence Analysis | Zendy

Peter Orszag | Zendy; Claudia Disqué | Zendy; Sonja Keim | Zendy; Michael G. Lorenz | Zendy; Olaf Wiesner | Zendy; Johannes Hadem | Zendy; Meike Stiesch | Zendy; Axel Haverich | Zendy; C. Kühn | Zendy

Open Access

Monitoring of Patients Supported by Extracorporeal Membrane Oxygenation for Systemic Infections by Broad-Range rRNA Gene PCR Amplification and Sequence Analysis

Author(s) -

Peter Orszag,

Claudia Disqué,

Sonja Keim,

Michael G. Lorenz,

Olaf Wiesner,

Johannes Hadem,

Meike Stiesch,

Axel Haverich,

C. Kühn

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02493-13

Subject(s) - extracorporeal membrane oxygenation , ribosomal rna , gene sequence , polymerase chain reaction , extracorporeal , biology , gene , sequence analysis , medicine , microbiology and biotechnology , 16s ribosomal rna , genetics

The rRNA gene PCR and sequencing test, SepsiTest, was compared with blood culture (BC) regarding the diagnosis of pathogens in 160 blood samples drawn from 28 patients during extracorporeal membrane oxygenation. With 45% of positive samples, SepsiTest was 13 to 75 h faster than BC. SepsiTest indicated bacteremias in 25% of patients who were BC negative.

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