Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli | Zendy

Tamara B. Souza | Zendy; Diego M. Lozer | Zendy; Sônia M. S. Kitagawa | Zendy; Liliana Cruz Spano | Zendy; Neusa P. Silva | Zendy; Isabel C. A. Scaletsky | Zendy

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Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli

Author(s) -

Tamara B. Souza,

Diego M. Lozer,

Sônia M. S. Kitagawa,

Liliana Cruz Spano,

Neusa P. Silva,

Isabel C. A. Scaletsky

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02478-12

Subject(s) - multiplex , multiplex polymerase chain reaction , escherichia coli , virulence , biology , microbiology and biotechnology , diarrhea , virology , polymerase chain reaction , gene , genetics , medicine

A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea.

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