SNaPBceBcon : a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans | Zendy

Ricardo Araújo | Zendy; Rita Caramalho | Zendy; Carla P. Coutinho | Zendy; Isabel SáCorreia | Zendy

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SNaPBceBcon : a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans

Author(s) -

Ricardo Araújo,

Rita Caramalho,

Carla P. Coutinho,

Isabel SáCorreia

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02476-15

Subject(s) - burkholderia , burkholderia cepacia complex , genotyping , genotype , biology , microbiology and biotechnology , typing , multilocus sequence typing , population , genetics , medicine , bacteria , gene , environmental health

We propose an optimized protocol for an extensive population analysis of Burkholderia cepacia and Burkholderia contaminans. Seven new polymorphisms were added to the recently proposed SNaPBcen assay, and a total of 18 markers ensured the clear identification and distinction of B. cepacia and B. contaminans isolates and high genotypic discrimination (Simpson index of 0.94) compared to those for multilocus sequence typing.

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