Comparing Pulsed-Field Gel Electrophoresis with Multilocus Sequence Typing, spa Typing, Staphylococcal Cassette Chromosome mec (SCC mec ) Typing, and PCR for Panton-Valentine Leukocidin, arcA , and opp3 in Methicillin-Resistant Staphylococcus aureus Isolates at a U.S. Medical Center

Methicillin-resistantStaphylococcus aureus (MRSA) has become a common cause of skin infections and invasive infections in community dwellers in the United States since the late 1990s. Isolates characterized as USA300 by pulsed-field gel electrophoresis (PFGE) are the predominant strain type in these infections. USA100 and USA500 strains commonly cause health care-associated infections. We compared PFGE with a number of other methods of genotyping in a sample of 149 clinical MRSA isolates from the University of Chicago Medical Center. The 5 USA500 isolates yielded 3spa types and 2 multilocus sequence types (MLSTs). Among the 24 USA100 isolates, 21 (88%) were ofspa type t002, 19 (79%) were of ST5, 2 carriedarcA andopp3 , and 1 was Panton-Valentine leukocidin positive (PVL+ ). Among the 102 USA300 isolates, 96 (94%) were of ST8 and 94 (92%) were ofspa type t008. The combination of traits that provided the best sensitivity (98%), specificity (97%), positive predictive value (PPV) (99%), and negative predictive value (NPV) (95%) for identifying USA300 isolates were the presence of thearcA gene and the presence of the PVL genes (area under the curve, 0.980; 95% confidence interval [CI], 0.955 to 1.0). PFGE did not delineate a homogeneous group of MRSA genetic backgrounds, as documented for other typing methods, particularly for USA500 and USA100 pulsotypes. Documenting the presence ofarcA and PVL genes by PCR was an efficient and accurate means of identifying USA300 in a collection of MRSA isolates in which USA300 is common. None of the tested genotyping methods provided an accurate means of identifying the next most common PFGE-based backgrounds, USA100 and USA500.