Quinupristin-Dalfopristin Resistance inEnterococcus faeciumIsolates from Humans, Farm Animals, and Grocery Store Meat in the United States | Zendy

Susan Donabedian | Zendy; Mary Beth Perri | Zendy; Dora Vager | Zendy; Ellie Hershberger | Zendy; Preeti Malani | Zendy; Shabbir Simjee | Zendy; Joseph W. Chow | Zendy; Emanuel N. Vergis | Zendy; R. R. Muder | Zendy; K. Gay | Zendy; Frederick J. Angulo | Zendy; Paul C. Bartlett | Zendy; Marcus Zervos | Zendy

Open Access

Quinupristin-Dalfopristin Resistance inEnterococcus faeciumIsolates from Humans, Farm Animals, and Grocery Store Meat in the United States

Author(s) -

Susan Donabedian,

Mary Beth Perri,

Dora Vager,

Ellie Hershberger,

Preeti Malani,

Shabbir Simjee,

Joseph W. Chow,

Emanuel N. Vergis,

R. R. Muder,

K. Gay,

Frederick J. Angulo,

Paul C. Bartlett,

Marcus Zervos

Publication year - 2006

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02412-05

Subject(s) - quinupristin , dalfopristin , enterococcus faecium , pulsed field gel electrophoresis , biology , microbiology and biotechnology , virginiamycin , enterococcus , veterinary medicine , gene , antibiotics , bacteria , genetics , genotype , vancomycin , medicine , staphylococcus aureus

Three hundred sixty-one quinupristin-dalfopristin (Q-D)-resistantEnterococcus faecium (QDREF) isolates were isolated from humans, turkeys, chickens, swine, dairy and beef cattle from farms, chicken carcasses, and ground pork from grocery stores in the United States from 1995 to 2003. These isolates were evaluated by pulsed-field gel electrophoresis (PFGE) to determine possible commonality between QDREF isolates from human and animal sources. PCR was performed to detect the streptogramin resistance genesvatD ,vatE , andvgbA and the macrolide resistance geneermB to determine the genetic mechanism of resistance in these isolates. QDREF from humans did not have PFGE patterns similar to those from animal sources.vatE was found in 35%, 26%, and 2% of QDREF isolates from turkeys, chickens, and humans, respectively, and was not found in QDREF isolates from other sources.ermB was commonly found in QDREF isolates from all sources. Known streptogramin resistance genes were absent in the majority of isolates, suggesting the presence of other, as-yet-undetermined, mechanisms of Q-D resistance.

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