RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA Test v1.5) for Early Diagnosis of HIV-1 Infection in Infants in Senegal | Zendy

Khadim Kébé | Zendy; Ousmane Ndiaye | Zendy; Halimatou DiopNdiaye | Zendy; P. Mbakob Mengue | Zendy; P. M. M. Guindo | Zendy; S. Diallo | Zendy; Nafissatou Lèye | Zendy; Sokhna Bousso Gueye | Zendy; AA Diallo | Zendy; C. Touré Kane | Zendy; Souleymane Mboup | Zendy

Open Access

RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA Test v1.5) for Early Diagnosis of HIV-1 Infection in Infants in Senegal

Author(s) -

Khadim Kébé,

Ousmane Ndiaye,

Halimatou DiopNdiaye,

P. Mbakob Mengue,

P. M. M. Guindo,

S. Diallo,

Nafissatou Lèye,

Sokhna Bousso Gueye,

AA Diallo,

C. Touré Kane,

Souleymane Mboup

Publication year - 2011

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02402-10

Subject(s) - venipuncture , virology , human immunodeficiency virus (hiv) , dried blood , viral load , whole blood , medicine , immunology , biology , chemistry , chromatography , anesthesia

The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMérieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection.

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