Open AccessRecognition of Streptococcuspseudoporcinus Colonization in Women as a Consequence of Using Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Group B Streptococcus Identification
Author(s) -
Nuntra Suwantarat,
Maureen Grundy,
Mayer Rubin,
Renee Harris,
Jo-Anne Miller,
Mark Romagnoli,
Ann Hanlon,
Tsigereda Tekle,
Brandon Ellis,
Frank R. Witter,
Karen C. Carroll
Publication year - 2015
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02363-15
Subject(s) - streptococcus , microbiology and biotechnology , streptococcus agalactiae , mass spectrometry , group b , urine , serotype , clinical microbiology , matrix assisted laser desorption/ionization , medicine , biology , bacteria , chromatography , chemistry , desorption , adsorption , genetics , organic chemistry
During a 14-month period of using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for group B streptococcus (GBS) identification, we recovered 32 (1%)Streptococcus pseudoporcinus isolates from 3,276 GBS screening cultures from female genital sources (25 isolates from pregnant women and 7 from nonpregnant women). An additional twoS. pseudoporcinus isolates were identified from a urine culture and a posthysterectomy wound culture. These isolates were found to cross-react with three different GBS antigen agglutination kits, PathoDx (Remel) (93%), Prolex (Pro-Lab Diagnostics) (38%), and Streptex (Remel) (53%). New approaches to bacterial identification in routine clinical microbiology laboratories may affect the prevalence ofS. pseudoporcinus .
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom