New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples | Zendy

Nicola Casson | Zendy; Klara M. PosfayBarbe | Zendy; Alain Gervaix | Zendy; Gilbert Greub | Zendy

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New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples

Author(s) -

Nicola Casson,

Klara M. PosfayBarbe,

Alain Gervaix,

Gilbert Greub

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02302-07

Subject(s) - biology , chlamydiales , bronchiolitis , polymerase chain reaction , dna , real time polymerase chain reaction , virology , pathology , gene , chlamydia , genetics , medicine , virus

Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.

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