Open AccessDevelopment of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Yersinia enterocolitica subsp. palearctica and Its Application to Bioserogroup 4/O3 Subtyping
Author(s) -
Rafał Gierczyński,
Andrey Golubov,
Heinrich Neubauer,
Jeannette N. Pham,
Alexander Rakin
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02252-06
Subject(s) - multiple loci vntr analysis , yersinia enterocolitica , subtyping , variable number tandem repeat , biology , genotyping , genotype , yersinia infections , tandem repeat , minisatellite , genetics , locus (genetics) , ribotyping , microbiology and biotechnology , microsatellite , escherichia coli , enterobacteriaceae , genome , bacteria , allele , gene , computer science , programming language
Yersinia enterocolitica bioserogroup 4/O3 is the predominant causative agent of yersiniosis in Europe and North America. Multiple-locus variable-number tandem-repeat analysis (MLVA) was developed to improve the resolution power of classical genotyping methods. MLVA based on six loci was able to distinguish 76 genotypes among 91Y. enterocolitica isolates of worldwide origin and 41 genotypes among 51 nonepidemiologically linked bioserogroup 4/O3 isolates, proving that it has a high resolution power. However, only a slight correlation of the MLVA genotypes and the geographic distribution of the isolates was observed. Although MLVA was also capable of distinguishing strains ofY. enterocolitica subsp.palearctica O9 and O5,27, there was only a minor correlation between the MLVA genotypes and serogroups. MLVA may be a helpful tool for epidemiological investigations ofY. enterocolitica subsp.palearctica outbreaks.