Open AccessSingle-Point Mutations Causing More than 100-Fold Underestimation of Human Immunodeficiency Virus Type 1 (HIV-1) Load with the Cobas TaqMan HIV-1 Real-Time PCR Assay
Author(s) -
Klaus Korn,
Benedikt Weißbrich,
Cornelia Henke-Gendo,
Albert Heim,
Christin Mariel Jauer,
Ni Taylor,
Josef Eberle
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02204-08
Subject(s) - virology , taqman , human immunodeficiency virus (hiv) , point mutation , biology , lentivirus , viral load , real time polymerase chain reaction , viral disease , mutation , genetics , gene
Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 Mgag sequences.