Single-Point Mutations Causing More than 100-Fold Underestimation of Human Immunodeficiency Virus Type 1 (HIV-1) Load with the Cobas TaqMan HIV-1 Real-Time PCR Assay | Zendy

Klaus Korn | Zendy; Benedikt Weißbrich | Zendy; Cornelia HenkeGendo | Zendy; Albert Heim | Zendy; Christin Mariel Jauer | Zendy; Ni Taylor | Zendy; Josef Eberle | Zendy

Open Access

Single-Point Mutations Causing More than 100-Fold Underestimation of Human Immunodeficiency Virus Type 1 (HIV-1) Load with the Cobas TaqMan HIV-1 Real-Time PCR Assay

Author(s) -

Klaus Korn,

Benedikt Weißbrich,

Cornelia HenkeGendo,

Albert Heim,

Christin Mariel Jauer,

Ni Taylor,

Josef Eberle

Publication year - 2009

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02204-08

Subject(s) - virology , taqman , human immunodeficiency virus (hiv) , point mutation , biology , lentivirus , viral load , real time polymerase chain reaction , viral disease , mutation , genetics , gene

Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 Mgag sequences.

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