Open AccessDevelopment and Evaluation of Immunochromatographic Assay for Simple and Rapid Detection of Campylobacter jejuni and Campylobacter coli in Human Stool Specimens
Author(s) -
Kentaro Kawatsu,
Yuko Kumeda,
Masumi Taguchi,
Wataru Yamazaki,
Masashi Kanki,
Kiyoshi Inoue
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02170-07
Subject(s) - campylobacter jejuni , campylobacter , microbiology and biotechnology , campylobacter coli , feces , escherichia coli , chromatography , bacteria , biology , chemistry , biochemistry , gene , genetics
An immunochromatographic assay (Campy-ICA) using a newly generated single monoclonal antibody against a 15-kDa cell surface protein ofCampylobacter jejuni was developed. When cell suspensions of 86C. jejuni strains and 27Campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the Campy-ICA, they all yielded positive results. The minimum detectable limits for theC. jejuni strains ranged from 1.8 × 104 to 8.2 × 105 CFU/ml of cell suspension, and those for theC. coli strains ranged from 1.4 × 105 to 4.6 × 106 CFU/ml of cell suspension. All 26 non-Campylobacter species tested yielded negative results with the Campy-ICA. To evaluate the ability of the Campy-ICA to detectC. jejuni andC. coli in human stool specimens, suspensions of 222 stool specimens from patients with acute gastroenteritis were treated with the bacterial protein extraction reagent, and the resulting extracts were tested with the Campy-ICA. The Campy-ICA results showed a sensitivity of 84.8% (28 of 33 specimens) and a specificity of 100% (189 of 189 specimens) compared to the results of isolation ofC. jejuni andC. coli from the stool specimens by a bacterial culture test. The Campy-ICA was simple to perform and was able to detectCampylobacter antigen in a fecal extract within 15 min. These results suggest that Campy-ICA testing of fecal extracts may be useful as a simple and rapid adjunct to stool culture for detectingC. jejuni andC. coli in human stool specimens.