Open AccessIs a Two-Step Glutamate Dehyrogenase Antigen-Cytotoxicity Neutralization Assay Algorithm Superior to the Premier Toxin A and B Enzyme Immunoassay for Laboratory Detection of Clostridium difficile ?
Author(s) -
Peter H. Gilligan
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02100-07
Subject(s) - immunoassay , toxin , clostridium difficile , neutralization , microbiology and biotechnology , cytotoxicity , clostridium difficile toxin a , clostridiaceae , clostridium difficile toxin b , enzyme , biology , virology , antibiotics , biochemistry , in vitro , immunology , antibody , virus
A two-step algorithm for the detection ofClostridium difficile by the use of C. Diff Quik Chek (TechLab, Blacksburg, VA) and a tissue culture cytotoxicity neutralization assay was found to be more sensitive than the widely used solid-phase enzyme immunoassay (EIA), the Premier toxin A and B EIA (Meridian Bioscience, Cincinnati, OH), and a newly developed, rapid single-test EIA forC. difficile toxins A and B (Tox A/B Quik Chek; TechLab).