16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures | Zendy

Ulrik Stenz Justesen | Zendy; Marianne Nielsine Skov | Zendy; Elisa Knudsen | Zendy; Hanne Marie Holt | Zendy; Per Søgaard | Zendy; T. Justesen | Zendy

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16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures

Author(s) -

Ulrik Stenz Justesen,

Marianne Nielsine Skov,

Elisa Knudsen,

Hanne Marie Holt,

Per Søgaard,

T. Justesen

Publication year - 2010

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02075-09

Subject(s) - 16s ribosomal rna , biology , bacteria , microbiology and biotechnology , ribosomal rna , anaerobic bacteria , identification (biology) , anaerobic exercise , gene , dna sequencing , genetics , physiology , botany

A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.

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