Open Access16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures
Author(s) -
Ulrik Stenz Justesen,
Marianne Nielsine Skov,
Elisa Knudsen,
Hanne Marie Holt,
Per Søgaard,
T. Justesen
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02075-09
Subject(s) - 16s ribosomal rna , biology , bacteria , microbiology and biotechnology , ribosomal rna , anaerobic bacteria , identification (biology) , anaerobic exercise , gene , dna sequencing , genetics , physiology , botany
A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.