Open AccessEvaluation of the “Dip Effect” Phenomenon in Antifungal Susceptibility Testing of Candida spp. against Echinocandins by Use of Gradient Concentration Strips
Author(s) -
Maria Siopi,
Marilena Tsala,
Nikolaos Siafakas,
Loukia Zerva,
Joseph Meletiadis
Publication year - 2015
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02073-15
Subject(s) - echinocandins , caspofungin , candida albicans , microbiology and biotechnology , candida glabrata , biology , candida tropicalis , antifungal , anidulafungin , amphotericin b
The “dip effect” phenomenon complicates antifungal susceptibility testing with gradient concentration strips. Of 60Candida isolates tested with the three echinocandins, this phenomenon was observed only for caspofungin with most (>90%)Candida albicans ,Candida glabrata , andCandida tropicalis isolates and for isolates with CLSI MICs of ≤0.25 mg/liter. In order to facilitate MIC determination, a practical approach was developed using the inhibition zones at 32, 8, 2, and 1 mg/liter, increasing the agreement with the CLSI method >86%.
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