Open AccessSimplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures
Author(s) -
Fernando Pasterán,
Nathalie Tijet,
Roberto G. Melano,
Alejandra Corso
Publication year - 2015
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02032-15
Subject(s) - microbiology and biotechnology , bacilli , biology , clinical microbiology , protocol (science) , bacterial protein , microbiological culture , bacteria , medicine , genetics , alternative medicine , pathology
We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPt-direct was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers.
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