Multilocus Phylogeny and Antifungal Susceptibility of Aspergillus Section Circumdati from Clinical Samples and Description of A. pseudosclerotiorum sp. nov | Zendy

João Paulo Zen Siqueira | Zendy; D.A. Sutton | Zendy; Josepa Gené | Zendy; Dania García | Zendy; Nathan P. Wiederhold | Zendy; Stephen W. Peterson | Zendy; Josep Guarro | Zendy

Open Access

Multilocus Phylogeny and Antifungal Susceptibility of Aspergillus Section Circumdati from Clinical Samples and Description of A. pseudosclerotiorum sp. nov

Author(s) -

João Paulo Zen Siqueira,

D.A. Sutton,

Josepa Gené,

Dania García,

Nathan P. Wiederhold,

Stephen W. Peterson,

Josep Guarro

Publication year - 2017

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.02012-16

Subject(s) - caspofungin , anidulafungin , micafungin , biology , posaconazole , voriconazole , internal transcribed spacer , terbinafine , itraconazole , amphotericin b , microbiology and biotechnology , aspergillosis , phylogenetic tree , antifungal , botany , genetics , gene

A multilocus phylogenetic study was carried out to assess species identity of a set of 34 clinical isolates fromAspergillus sectionCircumdati from the United States and to determine theirin vitro antifungal susceptibility against eight antifungal drugs. The genetic markers used were the internal transcribed spacer (ITS) region, and fragments of the beta-tubulin (BenA ), calmodulin (CaM ), and RNA polymerase II second largest subunit (RPB2 ) genes. The drugs tested were amphotericin B, itraconazole, posaconazole, voriconazole, anidulafungin, caspofungin, micafungin, and terbinafine. The most common species sampled wasA. westerdijkiae (29.4%), followed by a novel species, which was described here asA. pseudosclerotiorum (23.5%). Other species identified wereA. sclerotiorum (17.6%),A. ochraceus (8.8%),A. subramanianii (8.8%), andA. insulicola andA. ochraceopetaliformis , with two isolates (5.9%) of each. The drugs that showed the most potent activity were caspofungin, micafungin, and terbinafine, while amphotericin B showed the least activity.

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