Isolation and Biological and Molecular Characterization of Toxoplasma gondii from Canine Cutaneous Toxoplasmosis in Brazil
Author(s) -
Hilda Fátima Jesus Pena,
Ludmila Rodrigues Moroz,
Rita K. B. Sozigan,
Daniel Ajzenberg,
Fernando R. Carvalho,
Caroline M. Mota,
Tiago Wilson Patriarca Mineo,
Arlei Marcili
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02001-14
Subject(s) - toxoplasma gondii , toxoplasmosis , genotype , restriction fragment length polymorphism , biology , virulence , genotyping , virology , isolation (microbiology) , microsatellite , polymorphism (computer science) , microbiology and biotechnology , antibody , immunology , allele , genetics , gene
Cutaneous toxoplasmosis is a rare manifestation. This study represents a case report of an immunosuppressed dog that developed nodular dermal lesions caused by Toxoplasma gondii. The isolate (TgDgBr20) was characterized as mouse virulent and was genotyped as type BrI (ToxoDB genotype 6) using PCR-restriction fragment length polymorphism (RFLP) and as Africa 1 through microsatellite analysis.
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