Open AccessPCR-Restriction Fragment Length Polymorphism for Rapid, Low-Cost Identification of Isoniazid-Resistant Mycobacterium tuberculosis
Author(s) -
Maxine Caws,
Dau Quang Tho,
Minh-Duy Phan,
Nguyễn Thị Ngọc Lan,
Dai Viet Hoa,
M. Estée Török,
Tran Thi Hong Chau,
Nguyễn Văn Vĩnh Châu,
Nguyen Tran Chinh,
Jeremy Farrar
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01960-06
Subject(s) - isoniazid , mycobacterium tuberculosis , restriction fragment length polymorphism , tuberculosis , biology , microbiology and biotechnology , polymerase chain reaction , drug resistance , virology , medicine , genetics , gene , pathology
PCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-resistant katG_315 mutants. The test had a sensitivity of 80% and a specificity of 100% against conventional phenotypic drug susceptibility testing. The positive and negative predictive values were 1 and 0.86, respectively. None of the discrepant isolates had mutant katG_315 codons by sequencing. The test is cheap (less than $1.50 per test), specific, and suitable for the rapid identification of isoniazid resistance in regions with a high prevalence of katG_315 mutants among isoniazid-resistant M. tuberculosis isolates.