Open AccessDetection and Serotyping of Lyophilized Nonculturable Pneumococcal Isolates
Author(s) -
Joyanta K. Modak,
Mark C. Steinhoff,
Khalil Zaman,
Mohammad Shahidul Islam,
Shams El Arifeen,
Samir K. Saha
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01912-12
Subject(s) - serotype , microbiology and biotechnology , streptococcus pneumoniae , carriage , virology , pneumococcal infections , biology , pneumococcal vaccine , medicine , antibiotics , pathology
One hundred fifty-two nonculturable lyophilized carriage pneumococcal isolates from a vaccine trial were subjected to PCR for serotyping, and 149 (98%) were successfully classified as vaccine or nonvaccine types, which were similar to viable isolates. The methodology will be useful for analysis of this and other studies where stored pneumococcal isolates fail to grow.
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