Evaluation of Pneumococcal Serotyping by Multiplex PCR and Quellung Reactions | Zendy

Sandra S. Richter | Zendy; Kristopher P. Heilmann | Zendy; Cassie L. Dohrn | Zendy; Fathollah Riahi | Zendy; Daniel J. Diekema | Zendy; Gary V. Doern | Zendy

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Evaluation of Pneumococcal Serotyping by Multiplex PCR and Quellung Reactions

Author(s) -

Sandra S. Richter,

Kristopher P. Heilmann,

Cassie L. Dohrn,

Fathollah Riahi,

Daniel J. Diekema,

Gary V. Doern

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01876-13

Subject(s) - serotype , multiplex polymerase chain reaction , multiplex , microbiology and biotechnology , virology , streptococcus pneumoniae , pneumococcal infections , polymerase chain reaction , biology , genetics , antibiotics , gene

Screening of 1,750 pneumococcal isolates for common serotypes by PCR was followed by Quellung reaction analysis of PCR-negative isolates with a comparison to the conventional (Quellung reaction only) approach. PCR agreed with Quellung reaction results for 99% of isolates. The sequential PCR/Quellung reaction algorithm is accurate and more cost-effective than the conventional approach.

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