Prophages in Listeria monocytogenes Contain Single-Nucleotide Polymorphisms That Differentiate Outbreak Clones within Epidemic Clones
Author(s) -
Yi Chen,
Stephen J. Knabel
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01873-07
Subject(s) - prophage , listeria monocytogenes , biology , clone (java method) , outbreak , subtyping , single nucleotide polymorphism , genetics , genotype , virology , listeria , microbiology and biotechnology , bacteria , escherichia coli , dna , bacteriophage , gene , computer science , programming language
A fragment end ligation-mediated PCR strategy was used to analyze the AscI pulsed-field gel electrophoresis patterns of Listeria monocytogenes epidemic clone II (ECII), which led to the identification of single-nucleotide polymorphisms (SNPs) in prophage regions that differentiated the two ECII outbreak clones. SNPs in prophages that differentiated the outbreak clones of ECIII and -IV were also identified.
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