Development of a Flow Chart for Identification of Gram-Positive Anaerobic Cocci in the Clinical Laboratory | Zendy

Yuli Song | Zendy; Chengxu Liu | Zendy; Sydney M. Finegold | Zendy

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Development of a Flow Chart for Identification of Gram-Positive Anaerobic Cocci in the Clinical Laboratory

Author(s) -

Yuli Song,

Chengxu Liu,

Sydney M. Finegold

Publication year - 2006

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01872-06

Subject(s) - gram positive cocci , microbiology and biotechnology , biology , 16s ribosomal rna , anaerobic exercise , bacteroides , anaerobic bacteria , identification (biology) , bacteria , staphylococcus aureus , genetics , botany , physiology

Gram-positive anaerobic cocci (GPAC) are a heterogeneous group of organisms that are isolated from clinical specimens more often than any group of anaerobic bacteria except Bacteroides species, yet many strains are still difficult or impossible to identify in the diagnostic laboratory. In this study, a total of 124 strains, including 13 reference strains of GPAC species and 111 isolates that had been recovered from clinical specimens previously and identified by 16S rRNA gene sequencing, were subjected to biochemical characterization. Based on the results, a short biochemical scheme that involves the minimum essential biochemical tests for accurate identification of clinically important GPAC was developed.

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