Identification of Staphylococcus Species Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods | Zendy

Maitry S. Mehta | Zendy; Suzanne M. Paule | Zendy; Richard B. Thomson | Zendy; Karen L. Kaul | Zendy; Lance R. Peterson | Zendy

Open Access

Identification of Staphylococcus Species Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods

Author(s) -

Maitry S. Mehta,

Suzanne M. Paule,

Richard B. Thomson,

Karen L. Kaul,

Lance R. Peterson

Publication year - 2009

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01850-08

Subject(s) - staphylococcus , blood culture , staphylococcus aureus , microbiology and biotechnology , biology , micrococcaceae , melting curve analysis , species identification , bacteria , polymerase chain reaction , antibiotics , biochemistry , genetics , gene

We compared two real-time PCR assays (both by the use of melting curve analysis) for their ability to identifyStaphylococcus species directly from 200 positive blood culture bottles. The PCR assays correctly identified 83% to 94% of theStaphylococcus isolates to species clusters. Molecular testing significantly outperformed commercially available latex tests (sensitivity for both latex tests, <15%) when it was used directly with broth from signal-positive blood cultures.

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