Open AccessIdentification of Staphylococcus Species Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods
Author(s) -
Maitry S. Mehta,
Suzanne M. Paule,
Richard B. Thomson,
Karen L. Kaul,
Lance R. Peterson
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01850-08
Subject(s) - staphylococcus , blood culture , staphylococcus aureus , microbiology and biotechnology , biology , micrococcaceae , melting curve analysis , species identification , bacteria , polymerase chain reaction , antibiotics , biochemistry , genetics , gene
We compared two real-time PCR assays (both by the use of melting curve analysis) for their ability to identifyStaphylococcus species directly from 200 positive blood culture bottles. The PCR assays correctly identified 83% to 94% of theStaphylococcus isolates to species clusters. Molecular testing significantly outperformed commercially available latex tests (sensitivity for both latex tests, <15%) when it was used directly with broth from signal-positive blood cultures.