Open AccessSimultaneous Detection and Differentiation between Wild-Type and Vaccine Measles Viruses by a Multiplex Real-Time Reverse Transcription-PCR Assay
Author(s) -
Kanti Pabbaraju,
Kara Gill,
Anita Wong,
Graham Tipples,
Joanne Hiebert,
Alberto Severini,
Kevin Fonseca,
Raymond Tellier
Publication year - 2019
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01828-18
Subject(s) - virology , measles virus , measles , biology , multiplex , outbreak , morbillivirus , paramyxoviridae , mononegavirales , reverse transcription polymerase chain reaction , genotype , real time polymerase chain reaction , virus , multiplex polymerase chain reaction , microbiology and biotechnology , viral disease , polymerase chain reaction , gene , vaccination , genetics , gene expression
Measles is one of the most contagious viral respiratory infections and was declared to be eliminated from Canada in 1998; however, measles cases and outbreaks still occur every year through reintroduction from other parts of the world. Laboratory confirmation of measles virus (MV) RNA by real-time PCR provides a definitive diagnosis, and molecular analysis to determine the genotype is the only way to distinguish between wild-type and vaccine strains.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom