Open AccessDevelopment of the TypeSeq Assay for Detection of 51 Human Papillomavirus Genotypes by Next-Generation Sequencing
Author(s) -
Sarah Wagner,
David A. Roberson,
Joseph F. Boland,
Meredith Yeager,
Michael Cullen,
Lisa Mirabello,
S. Terence Dunn,
Joan L. Walker,
Rosemary E. Zuna,
Mark Schiffman,
Nicolas Wentzensen
Publication year - 2019
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01794-18
Subject(s) - genotype , human papillomavirus , biology , virology , genetics , computational biology , medicine , gene
We have developed a new human papillomavirus (HPV) genotyping assay for detection of 51 HPV genotypes by next-generation sequencing (NGS). The TypeSeq assay consists of 3 PCR steps that equalize viral load and each type’s amplicon copies prior to genotyping by NGS, thereby maximizing multiple-type sensitivity with minimal sequencing reads.
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