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The multiplexed immunoassay (MIA) is an automated, monoclonal antibody-based serotyping assay that uses culture lysates ofStreptococcus pneumoniae . This study describes the development and validation of applying MIA directly to sputum samples for the serotype-specific detection ofS. pneumoniae . Sputum optimization involved liquefaction and fractionation.

journal of clinical microbiology

Development for Clinical Use of a Multiplexed Immunoassay Using Sputum Samples for Streptococcus pneumoniae: a Non-Culture-Based Approach for Serotype-Specific Detection