Open AccessDevelopment for Clinical Use of a Multiplexed Immunoassay Using Sputum Samples for Streptococcus pneumoniae: a Non-Culture-Based Approach for Serotype-Specific Detection
Author(s) -
Sun Jin Kim,
Yoo Jung Jeong,
Jong Hun Kim,
Young Kyung Yoon,
Jang Wook Sohn,
Moon H. Nahm,
Min Ja Kim
Publication year - 2019
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01782-18
Subject(s) - streptococcus pneumoniae , immunoassay , sputum , serotype , microbiology and biotechnology , monoclonal antibody , sputum culture , biology , virology , medicine , antibody , immunology , antibiotics , pathology , tuberculosis
The multiplexed immunoassay (MIA) is an automated, monoclonal antibody-based serotyping assay that uses culture lysates ofStreptococcus pneumoniae . This study describes the development and validation of applying MIA directly to sputum samples for the serotype-specific detection ofS. pneumoniae . Sputum optimization involved liquefaction and fractionation.
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