Open AccessDetection of Heteroresistant Mycobacterium tuberculosis by Pyrosequencing
Author(s) -
A. Engström,
Sven Hoffner,
Pontus Juréen
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01761-13
Subject(s) - pyrosequencing , mycobacterium tuberculosis , tuberculosis , biology , microbiology and biotechnology , population , mycobacterium , mycobacterium tuberculosis complex , mutant , dna , virology , genetics , bacteria , medicine , gene , pathology , environmental health
The ability of pyrosequencing to detect a resistant minority population of a heteroresistant Mycobacterium tuberculosis strain was investigated by performing a titration study. A mutant signal was noted only when the proportion of mutant DNA in the DNA target was 35 to 50%, showing that the sensitivity is significantly lower than that of phenotypic drug susceptibility test methods.
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