Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study | Zendy

ChoonMee Kim | Zendy; Min Keun Cho | Zendy; DongMin Kim | Zendy; Na-Ra Yun | Zendy; Seok Won Kim | Zendy; Sook Jin Jang | Zendy; YoungJoon Ahn | Zendy; Dong-Hoon Lim | Zendy

Open Access

Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

Author(s) -

ChoonMee Kim,

Min Keun Cho,

DongMin Kim,

Na-Ra Yun,

Seok Won Kim,

Sook Jin Jang,

YoungJoon Ahn,

Dong-Hoon Lim

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01754-15

Subject(s) - 16s ribosomal rna , scrub typhus , biology , ribosomal rna , gene , polymerase chain reaction , virology , microbiology and biotechnology , genetics

We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.

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